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KMID : 0359320170570010031
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Korean Journal of Veterinary Research
2017 Volume.57 No. 1 p.31 ~ p.36
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Improvement of indirect enzyme-linked immunosorbent assay for detection of Japanese encephalitis virus antibodies in swine sera
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Yang Dong-Kun
Kim Ha-Hyun
Jo Hyun-Ye
Lee Seung-Heon
Jang Sang-Ho
Lee Sang-Oh
Choi Sung-Suk
Cho In-Soo
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Abstract
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Japanese encephalitis (JE) is an important zoonosis caused by the mosquito-transmitted JE virus (JEV), which is a causative agent of reproductive failure in pregnant sows. Detection of JEV antibodies in swine is performed by hemagglutination inhibition (HI), virus neutralization (VN), and the plaque reduction neutralization test (PRNT). The most stringent PRNT is the 90% endpoint PRNT (PRNT90). These conventional assays are difficult to carry out in diagnostic laboratories with insufficient instruments or cell culture systems. An alternative assay that is easily conducted and time efficient is required. In this study, we improved the indirect enzyme-linked immunosorbent assay (I-ELISA) with clarified antigen for the detection of JEV antibodies. The I-ELISA results obtained from 175 swine serum samples were compared with HI, VN, and PRNT90 results. The sensitivity of I-ELISA was 91.8%, 95.0%, and 94.7% compared with HI, VN, and PRNT90 results, respectively. The specificity of I-ELISA was 92.2%, 94.7%, and 94.7% compared with HI, VN, and PRNT90 results, respectively. Moreover, the I-ELISA results were significantly correlated with the HI (r = 0.93), VN (r = 0.95), and PRNT90 (r = 0.92) results. These results suggest that the improved I-ELISA is useful for serosurveillance of JEV in swine.
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KEYWORD
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Japanese encephalitis virus, indirect enzyme-linked immunosorbent assay, surveillance, swine
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